Please note: While some information will still be current in a year, other information may already be out of date in three months time. If you are in any doubt, please feel free to ask.

Dornase alpha

How is dornase alpha prepared or made? I have read that it is recombinant dna but from which cells is it taken?
Dear questioner,
after contacting the pharmaceutical company Roche producing Pulmozyme® I can provide you with the following information: the product contains the human enzyme dornase alpha. Genetic information for human dornase alpha its transferred to cells from the ovaries of the Chinese hamster A 14,16 – 1 MSB #757, that produce in the sequel human Desoxyribonuclease I or recombinant dornase alpha. This method is called recombinant DNA-technology.
Hope to have answered your question,
Best regards,
Dr. Daniela d’Alquen (Coordinator of the Central English Archive of ECORN-CF)
The answer is edited by: Prof Judy Bradley